10.1371/journal.pone.0096286.g005
Jack H. Wang
Jack
H. Wang
Kosei Eguchi
Kosei
Eguchi
Sahohime Matsumoto
Sahohime
Matsumoto
Katsuhito Fujiu
Katsuhito
Fujiu
Issei Komuro
Issei
Komuro
Ryozo Nagai
Ryozo
Nagai
Ichiro Manabe
Ichiro
Manabe
EPA reduces <i>Mmp9</i> expression in macrophages.
Public Library of Science
2014
anatomy
Cardiovascular anatomy
Biochemistry
lipids
Fatty acids
cell biology
Cellular types
Animal cells
Blood cells
White blood cells
monocytes
Immune cells
immunology
cardiology
Cardiovascular diseases
Valvular diseases
Diagnostic medicine
Diagnostic radiology
tomography
Computed axial tomography
Radiology and imaging
Vascular medicine
Aortic diseases
Model organisms
Animal models
Mouse models
2014-05-05 03:30:16
Figure
https://plos.figshare.com/articles/figure/_EPA_reduces_Mmp9_expression_in_macrophages_/1016205
<p><b>A</b>. Gelatin zymography of aortic tissues one week after CaCl<sub>2</sub> treatment together with quantitative analysis, showing reduced MMP9 activity in samples from the EPA diet group. Equal amounts of protein (20 µg) were loaded per aortic sample. For quantitation, <i>n</i> = 6–7 in each group. <b>B</b>. Gating strategy for the flow cytometric analysis of AAA macrophages. Macrophages were identified as Ly-6C<sup>low</sup>CD11b<sup>+</sup>F4/80<sup>+</sup>Ly-6G<sup>−</sup> cells (full gating strategy shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096286#pone.0096286.s001" target="_blank">Figure S2 in File S1</a>). <b>C</b>. The number of aortic macrophages per aortic sample. No statistically significant difference in the number of aortic macrophages between control diet and EPA diet groups was detected. <b>D</b>. The mRNA levels of <i>Mmp9</i> in sorted aortic macrophages. Expression levels were first normalized to <i>18s</i> rRNA levels and then further normalized to the level of control diet group. <i>n</i> = 5 in each group. <b>E</b>. RAW264.7 macrophages were cultured with either vehicle (10% BSA) or EPA (50 µmol/L) for 48 hours. The cells were then stimulated with recombinant mouse TNF-α (20 ng/mL) for a further 6 hours and harvested for analysis by RT-PCR. Expression levels were first normalized to <i>18s</i> rRNA levels and then presented as relative expression compared to baseline vehicle sample. <i>n</i> = 3 per condition. *<i>P</i><0.05 compared to control diet group in <b>A</b> and <b>D</b> or respective vehicle controls in <b>E</b>.</p>