Glaser, Talita La Banca de Oliveira, Sophia Cheffer, Arquimedes Beco, Renata Martins, Patrícia Fornazari, Maynara Lameu, Claudiana Miranda Costa Junior, Helio Coutinho-Silva, Robson Ulrich, Henning Immunofluorescence studies of P2X7 receptor and differentiation-stage specific proteins in undifferentiated and differentiated ESC. <p>P2X7R expression and co-localization with differentiation-stage specific proteins was determined by confocal microscopy and immunofluorescence assays as described in Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096281#s2" target="_blank">Methods</a>. (<b>A</b>). Left panel: Co-localization between SSEA-1 (stage-specific embryonic antigen-1) and P2X7R immunofluorescence in undifferentiated ES cells. Co-localization of the protein is shown by Z-stack analysis. Right panel: Double-immunostaining for nestin (neural stem and precursor marker) and P2X7R expression in cells induced to differentiation for 8 days. (<b>B</b>). Left panel: Immunostaining for Oct-4 (pluripotency marker) in undifferentiated cells. Middle panel: Immunostaining for Oct-4 and SSEA-1 in undifferentiated cells. Right panel: Staining pattern for the neuron-specific marker β-tubulin in neural-differentiated cells. Cell nuclei were visualized by DAPI staining. Scale bar, 50 µm.</p> cell biology;Cell processes;Cell growth;Cellular types;Animal cells;stem cells;Embryonic stem cells;Signal transduction;cell signaling;Developmental signaling;Molecular cell biology;developmental biology;Cell differentiation;Cell fate determination;Molecular development;genetics;gene expression;neuroscience;Developmental neuroscience;studies;p2x7;receptor;differentiation-stage;proteins;undifferentiated;differentiated 2014-05-05
    https://plos.figshare.com/articles/figure/_Immunofluorescence_studies_of_P2X7_receptor_and_differentiation_stage_specific_proteins_in_undifferentiated_and_differentiated_ESC_/1016200
10.1371/journal.pone.0096281.g003