%0 Figure %A Glaser, Talita %A La Banca de Oliveira, Sophia %A Cheffer, Arquimedes %A Beco, Renata %A Martins, Patrícia %A Fornazari, Maynara %A Lameu, Claudiana %A Miranda Costa Junior, Helio %A Coutinho-Silva, Robson %A Ulrich, Henning %D 2014 %T Immunofluorescence studies of P2X7 receptor and differentiation-stage specific proteins in undifferentiated and differentiated ESC. %U https://plos.figshare.com/articles/figure/_Immunofluorescence_studies_of_P2X7_receptor_and_differentiation_stage_specific_proteins_in_undifferentiated_and_differentiated_ESC_/1016200 %R 10.1371/journal.pone.0096281.g003 %2 https://ndownloader.figshare.com/files/1484811 %K cell biology %K Cell processes %K Cell growth %K Cellular types %K Animal cells %K stem cells %K Embryonic stem cells %K Signal transduction %K cell signaling %K Developmental signaling %K Molecular cell biology %K developmental biology %K Cell differentiation %K Cell fate determination %K Molecular development %K genetics %K gene expression %K neuroscience %K Developmental neuroscience %K studies %K p2x7 %K receptor %K differentiation-stage %K proteins %K undifferentiated %K differentiated %X

P2X7R expression and co-localization with differentiation-stage specific proteins was determined by confocal microscopy and immunofluorescence assays as described in Materials and Methods. (A). Left panel: Co-localization between SSEA-1 (stage-specific embryonic antigen-1) and P2X7R immunofluorescence in undifferentiated ES cells. Co-localization of the protein is shown by Z-stack analysis. Right panel: Double-immunostaining for nestin (neural stem and precursor marker) and P2X7R expression in cells induced to differentiation for 8 days. (B). Left panel: Immunostaining for Oct-4 (pluripotency marker) in undifferentiated cells. Middle panel: Immunostaining for Oct-4 and SSEA-1 in undifferentiated cells. Right panel: Staining pattern for the neuron-specific marker β-tubulin in neural-differentiated cells. Cell nuclei were visualized by DAPI staining. Scale bar, 50 µm.

%I PLOS ONE