Cell-Membrane-Anchored
DNA Nanoplatform for Programming
Cellular Interactions
Jin Li
Kanyu Xun
Ke Pei
Xiaojing Liu
Xueyu Peng
Yulin Du
Liping Qiu
Weihong Tan
10.1021/jacs.9b04725.s001
https://acs.figshare.com/articles/journal_contribution/Cell-Membrane-Anchored_DNA_Nanoplatform_for_Programming_Cellular_Interactions/10124123
Cell–cell
interactions are mediated through compositions
expressed on the membrane. Engineering the cell surface to display
functional modules with high biocompatibility, high controllability,
and high stability would offer great opportunities for studying and
manipulating these intercellular reactions. However, it remains a
technical challenge because of the complex and dynamic nature of the
cell membrane. Herein, by using three-dimensional (3D) amphiphilic
pyramidal DNA as the scaffold, we develop a biocompatible, effective,
and versatile strategy for engineering the cell surface with DNA probes.
Compared with linear DNA constructs, these pyramidal probes show higher
(nearly 100-fold) membrane-anchoring stability and higher (about 2.5-fold)
target accessibility. They enable specific, effective, and tunable
connections between cells. Meanwhile, our results indicate that connecting
cells in close proximity are critical to initiate intercellular communication.
By combining high programmability and high diversity of DNA probes,
this strategy is expected to provide a powerful and designable membrane-anchored
nanoplatform for studying multicellular communication networks.
2019-11-01 14:33:51
designable membrane-anchored nanoplatform
stability
cell surface
Cell-Membrane-Anchored DNA Nanoplatform
DNA probes
strategy
intercellular
multicellular communication networks