Manes, Gaël Cheguru, Pallavi Majumder, Anurima Bocquet, Béatrice Sénéchal, Audrey Artemyev, Nikolai O. P. Hamel, Christian Brabet, Philippe Expression of PDE6β1-314fs*50 in transgenic <i>X. laevis</i> rods photoreceptors. <p>A. <i>left</i> - EGFP fluorescence in living photoreceptor cells expressing PDE6β1-314fs*50, <i>center</i> - DIC image, <i>right</i> - EGFP-fluorescence/DIC overlay. <i>Bar</i> - 10 µm. B. Co-localization of PDE6β1-314fs*50 (green) with the rod outer segment marker Wheat Germ Agglutinin (red) in cryosections of transgenic retina. Cryosections were counterstained with TO-PRO3 nuclear stain (blue). <i>Bar</i> - 10 µm. C. Isotonic (I), hypotonic (H) extracts of transgenic PDE6β1-314fs*50 retinas were analyzed by immunoblotting with anti-GFP B-2 monoclonal antibody.</p> agriculture;Agricultural biotechnology;Genetically modified organisms;Transgenic animals;Biochemistry;proteins;genetics;mutation;neuroscience;Sensory systems;Visual system;organisms;animals;vertebrates;amphibians;frogs;xenopus;ophthalmology;Inherited eye disorders;Retinal disorders;Model organisms;Animal models;transgenic;rods 2014-04-23
    https://plos.figshare.com/articles/figure/_Expression_of_PDE6_1_314fs_50_in_transgenic_X_laevis_rods_photoreceptors_/1005885
10.1371/journal.pone.0095768.g005