Figure 4 Mary O′Connell Motherway Debbie Watson Francesca Bottacini Tyson A. Clark Richard J. Roberts Jonas Korlach Peggy Garault Christian Chervaux Johan E. T. van Hylckama Vlieg Tamara Smokvina Douwe van Sinderen 10.1371/journal.pone.0094875.g004 https://plos.figshare.com/articles/figure/_Figure_4_/1003561 <p><b>(A)</b>. Restriction analysis of total DNA from <i>B. animalis</i> subsp. <i>lactis</i> CNCM I-2494. Lane1, molecular weight marker X (Roche). Lane 2: Unrestricted total DNA from <i>B. animalis</i> subsp. <i>lactis</i> CNCM I-2494, Lane 3–5 total <i>B. animalis</i> subsp. <i>lactis</i> CNCM I-2494 DNA restricted with lane 3, HindIII; lane 4, AvaII and lane 5, EcoRI. <b>(B)</b> Restriction analysis of plasmid DNA isolated from three representative <i>E. coli</i> Xl1Blue -pWSK29-M.BanLII. Plasmid DNA from <i>E. coli</i> Xl1Blue -pWSK29-M.BanLII restricted with PstI and XbaI, lanes 2–4 (expected and obtained, product sizes of PstI +XbaI digests are 5420 bp and 1605 bp), or AvaII, lanes 5–7.</p> 2014-04-17 04:13:19 molecular biology Molecular biology techniques cloning genetics genomics Functional genomics gene expression