Verrucarin A inhibits SRC-3 downstream target gene (MMP2 and MMP13) expression.
Fei Yan
Yang Yu
Dar-Chone Chow
Timothy Palzkill
Franck Madoux
Peter Hodder
Peter Chase
Patrick R. Griffin
Bert W. O'Malley
David M. Lonard
10.1371/journal.pone.0095243.g004
https://plos.figshare.com/articles/figure/_Verrucarin_A_inhibits_SRC_3_downstream_target_gene_MMP2_and_MMP13_expression_/1003559
<p>(A–B) Luciferase assays were performed in HeLa cells transiently transfected with MMP2-Luc, MMP13-Luc, and pCR3.1-SRC-3 expression vectors before incubation with verrucarin A at different concentrations (0, 2, 5, and 10 nM) for 24 h. (C) H1299 cells were treated with verrucarin A at different concentrations (0, 10, and 20 nM) for 24 h, then real-time PCR was performed to quantitate SRC-1, SRC-2, SRC-3, MMP2, and MMP13 mRNA expression.</p>
2014-04-17 04:12:05
Biochemistry
enzymology
Enzyme chemistry
Cofactors (biochemistry)
chemical biology
biotechnology
Small molecules
oncology
Cancer treatment
pharmacology
Drug research and development
drug discovery
inhibits
src-3
downstream