Verrucarin A inhibits SRC-3 downstream target gene (MMP2 and MMP13) expression. Fei Yan Yang Yu Dar-Chone Chow Timothy Palzkill Franck Madoux Peter Hodder Peter Chase Patrick R. Griffin Bert W. O'Malley David M. Lonard 10.1371/journal.pone.0095243.g004 https://plos.figshare.com/articles/figure/_Verrucarin_A_inhibits_SRC_3_downstream_target_gene_MMP2_and_MMP13_expression_/1003559 <p>(A–B) Luciferase assays were performed in HeLa cells transiently transfected with MMP2-Luc, MMP13-Luc, and pCR3.1-SRC-3 expression vectors before incubation with verrucarin A at different concentrations (0, 2, 5, and 10 nM) for 24 h. (C) H1299 cells were treated with verrucarin A at different concentrations (0, 10, and 20 nM) for 24 h, then real-time PCR was performed to quantitate SRC-1, SRC-2, SRC-3, MMP2, and MMP13 mRNA expression.</p> 2014-04-17 04:12:05 Biochemistry enzymology Enzyme chemistry Cofactors (biochemistry) chemical biology biotechnology Small molecules oncology Cancer treatment pharmacology Drug research and development drug discovery inhibits src-3 downstream