10.1371/journal.pone.0095243.g004 Fei Yan Fei Yan Yang Yu Yang Yu Dar-Chone Chow Dar-Chone Chow Timothy Palzkill Timothy Palzkill Franck Madoux Franck Madoux Peter Hodder Peter Hodder Peter Chase Peter Chase Patrick R. Griffin Patrick R. Griffin Bert W. O'Malley Bert W. O'Malley David M. Lonard David M. Lonard Verrucarin A inhibits SRC-3 downstream target gene (MMP2 and MMP13) expression. Public Library of Science 2014 Biochemistry enzymology Enzyme chemistry Cofactors (biochemistry) chemical biology biotechnology Small molecules oncology Cancer treatment pharmacology Drug research and development drug discovery inhibits src-3 downstream 2014-04-17 04:12:05 Figure https://plos.figshare.com/articles/figure/_Verrucarin_A_inhibits_SRC_3_downstream_target_gene_MMP2_and_MMP13_expression_/1003559 <p>(A–B) Luciferase assays were performed in HeLa cells transiently transfected with MMP2-Luc, MMP13-Luc, and pCR3.1-SRC-3 expression vectors before incubation with verrucarin A at different concentrations (0, 2, 5, and 10 nM) for 24 h. (C) H1299 cells were treated with verrucarin A at different concentrations (0, 10, and 20 nM) for 24 h, then real-time PCR was performed to quantitate SRC-1, SRC-2, SRC-3, MMP2, and MMP13 mRNA expression.</p>