ATHB17Δ113 can bind both Class II and Class I DNA targets and ATHB17Δ113 containing V182A-Q185A-N186A mutation cannot bind Class II DNA target in <i>in vitro</i> assay (measured by Surface Plasmon Resonance (SPR).
Elena A. Rice
Abha Khandelwal
Robert A. Creelman
Cara Griffith
Jeffrey E. Ahrens
J. Philip Taylor
Lesley R. Murphy
Siva Manjunath
Rebecca L. Thompson
Matthew J. Lingard
Stephanie L. Back
Huachun Larue
Bonnie R. Brayton
Amanda J. Burek
Shiv Tiwari
Luc Adam
James A. Morrell
Rico A. Caldo
Qing Huai
Jean-Louis K. Kouadio
Rosemarie Kuehn
Anagha M. Sant
William J. Wingbermuehle
Rodrigo Sala
Matt Foster
Josh D. Kinser
Radha Mohanty
Dongming Jiang
Todd E. Ziegler
Mingya G. Huang
Saritha V. Kuriakose
Kyle Skottke
Peter P. Repetti
T. Lynne Reuber
Thomas G. Ruff
Marie E. Petracek
Paul J. Loida
10.1371/journal.pone.0094238.t001
https://plos.figshare.com/articles/dataset/_ATHB17_113_can_bind_both_Class_II_and_Class_I_DNA_targets_and_ATHB17_113_containing_V182A_Q185A_N186A_mutation_cannot_bind_Class_II_DNA_target_in_in_vitro_assay_measured_by_Surface_Plasmon_Resonance_SPR_/1000569
<p>Binding affinities and Kinetic constants of ATHB17Δ113 interacting with Class I and Class II type DNA, measured by Biacore 2000, globally fitted. SPR measurements with Biacore 2000 were at 25°C in HBS-EP, 100 ug/ml BSA (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20,100 ug/ml BSA). Equilibrium dissociation constant K<sub>D</sub> = k<sub>off</sub>/k<sub>on.</sub></p>
2014-04-15 04:30:42
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ii
dna
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containing
v182a-q185a-n186a
mutation
cannot
assay
plasmon
resonance