ATHB17Δ113 can bind both Class II and Class I DNA targets and ATHB17Δ113 containing V182A-Q185A-N186A mutation cannot bind Class II DNA target in <i>in vitro</i> assay (measured by Surface Plasmon Resonance (SPR). Elena A. Rice Abha Khandelwal Robert A. Creelman Cara Griffith Jeffrey E. Ahrens J. Philip Taylor Lesley R. Murphy Siva Manjunath Rebecca L. Thompson Matthew J. Lingard Stephanie L. Back Huachun Larue Bonnie R. Brayton Amanda J. Burek Shiv Tiwari Luc Adam James A. Morrell Rico A. Caldo Qing Huai Jean-Louis K. Kouadio Rosemarie Kuehn Anagha M. Sant William J. Wingbermuehle Rodrigo Sala Matt Foster Josh D. Kinser Radha Mohanty Dongming Jiang Todd E. Ziegler Mingya G. Huang Saritha V. Kuriakose Kyle Skottke Peter P. Repetti T. Lynne Reuber Thomas G. Ruff Marie E. Petracek Paul J. Loida 10.1371/journal.pone.0094238.t001 https://plos.figshare.com/articles/dataset/_ATHB17_113_can_bind_both_Class_II_and_Class_I_DNA_targets_and_ATHB17_113_containing_V182A_Q185A_N186A_mutation_cannot_bind_Class_II_DNA_target_in_in_vitro_assay_measured_by_Surface_Plasmon_Resonance_SPR_/1000569 <p>Binding affinities and Kinetic constants of ATHB17Δ113 interacting with Class I and Class II type DNA, measured by Biacore 2000, globally fitted. SPR measurements with Biacore 2000 were at 25°C in HBS-EP, 100 ug/ml BSA (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20,100 ug/ml BSA). Equilibrium dissociation constant K<sub>D</sub> = k<sub>off</sub>/k<sub>on.</sub></p> 2014-04-15 04:30:42 agriculture Agricultural biotechnology Genetically modified organisms Transgenic plants crops Cereal crops maize biotechnology Genetic engineering Transgenic engineering Plant biotechnology cell biology Molecular cell biology genetics gene expression Plant genetics Plant science ii dna targets containing v182a-q185a-n186a mutation cannot assay plasmon resonance