(A) A SDS-PAGE showing the purified recombinant preS

Copyright information:

Taken from "Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients"

http://www.virologyj.com/content/4/1/93

Virology Journal 2007;4():93-93.

Published online 24 Sep 2007

PMCID:PMC2082030.

(B) Binding of HBsAg to anti-HBs and to polyclonal anti-preS. An ELISA Kit for HBsAg detection was used to detect HBsAg in the serum sample of an HBeAg positive patient (black square). Recombinant S protein (white square) was used as control. Binding of HBsAg to polyclonal anti-preS (white circle) was detected by adding recombinant S protein to a 96-well microplate which was coated with the polyclonal anti-preS antibody, using the same HRP-anti-HBs in the HBsAg kit. The binding to polyclonal anti-preS by antigens in an HBeAg positive patient serum sample is shown as (black circle). The X axis is plotted in logarithm. The absorbance at 450 nm has been subtracted by the background.

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