Expression of Lin28a and Ddx4 in adult mouse testes following busulfan treatment.

Representative images showing immunohistochemical stainings for Lin28a (left column) and Ddx4 (right column) on testis sections of DMSO (A, B) and busulfan treated (C–L) adult mice. For Lin28a and Ddx4 stainings on days 1 (C), 3 (E), 7 (G), 21 (I) and 28 (K) after busulfan treatment higher magnifications are shown for each time point (D, F, H, J, L). Germ cells are indicated by arrows and Sertoli cells by arrow heads. As negative control, stainings with nonspecific IgGs were performed (A; Insert). Scale bars represent 25 µm and 50 µm.