WBSCR22 interacts with the TRMT112 protein in mammalian cells.

(A) WBSCR22 protein expression in U2OS and U2OS cell line stably expressing the E2Tag-WBSCR22 was determined by western blot using anti-WBSCR22 antibody. α-tubulin detected with anti-α-tubulin antibodies is used as a loading control. (B) Experimental scheme of the SILAC coupled co-immunoprecipitation assay performed. (C) The WBSCR22 protein interaction partners in U2OS cells stably expressing the WBSCR22 protein. H/L ratio shows the relative enrichment of the peptides of proteins pulled-down with WBSCR22 compared to mock control. (D) The WBSCR22 protein interacts with TRMT112. The WBSCR22 protein was immunoprecipitated from U2OS and U2OS-E2Tag-WBSCR22 cell lysates with an antibody against E2Tag. Proteins of the extract (Input; 10% of the lysate and pulled-down fraction (IP) were analyzed by immunoblotting with antibodies against WBSCR22, TRMT112 and C1QBP.