Figure_S1.tif (3.87 MB)
Figure S1 - Variable VlsE Is Critical for Host Reinfection by the Lyme Disease Spirochete
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posted on 2013-04-08, 02:07 authored by Artem S. Rogovskyy, Troy BankheadSDS-PAGE analysis of whole-cell lysates of B. burgdorferi clones. WT, sVlsE, and ΔVlsE whole-cell lysates (106 cells/lane) were subjected to electrophoresis in a 15% sodium dodecyl sulfate polyacrylamide gel under reducing conditions. Electrophoresis was carried out in Tris-glycine buffer containing 0.01% SDS. The slab gel was stained with Coomassie Blue R-250 and destained with methanol∶water∶acetic acid (5∶4∶1, v/v).
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