Tetracycline treatment models S. enterica acute infection in C. elegans.

(A) fer-1(b232ts) L1 animals were exposed to S. enterica—GFP for 72 hours. (B) fer-1(b232ts) L1 animals were exposed to S. enterica—GFP for 72 hours and transferred to the indicated plates for 24 hours. (C) fer-1(b232ts) L1 animals were exposed to S. enterica—GFP for 96 hours and transferred to the indicated plates for 24 hours. At each time point, overall GFP intensity in the intestinal lumen was determined using an MZFLIII Leica stereomicroscope. Three levels of colonization were determined as heavy, weak, or none as described in Materials and Methods. N = 104–150 animals per condition. The graph represents the combined results of 2 independent experiments. (D) Quantification of colony forming units of fer-1(b232ts) L1 animals exposed to S. enterica—GFP for 72 hours, S. enterica—GFP for 96 hours, or S. enterica—GFP for 72 hours and then treated with Tetracycline for 24 hours. N = 10 animals per condition. The graph represents the combined results of 4 independent experiments. (E) fer-1(b232ts) L1 animals were exposed to E. coli or S. enterica—GFP for 72 hours and then transferred to E. coli plus Tetracycline or S. enterica—GFP. Animals were scored for survival 72 hour post initial exposure to S. enterica. N = 60 animals per condition. The graph represents the combined results of 5 independent experiments.