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Reactivity of scFv-LT recombinant antibody against LT toxin.
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posted on 2015-07-08, 03:23 authored by Christiane Y. Ozaki, Caio R. F. Silveira, Fernanda B. Andrade, Roberto Nepomuceno, Anderson Silva, Danielle D. Munhoz, Bruno B. Yamamoto, Daniela Luz, Patrícia A. E. Abreu, Denise S. P. Q. Horton, Waldir P. Elias, Oscar H. P. Ramos, Roxane M. F. Piazza(A) Nitrocellulose membrane containing the LT toxin protein after SDS-PAGE 15% were subjected to immunoblotting with antibodies. Lanes: 1. scFv-LT 200 μg/mL, 2. 50 μg/mL anti-LT mAb. (B) Immunofluorescence assay after LT interaction with Y-1 cells. Panel 1. Reactivity with scFv-LT; Panel 2. Reactivity with anti-LT mAb. Panel 3. Reactivity with scFv-LT in the absence of LT toxin. Panel 4. Light microscopy. (C) scFv-LT titration with LT toxin by capture ELISA. (D) Detection of LT in culture supernatants of LT- and LT/ST-producing ETEC isolates by capture ELISA.
