Phylogenetic tree and comparison of Est16 with esterases/lipases showing their biotechnological applications.

(A) A phylogenetic tree of 18 sequences from patented esterases/lipases, the sequence of aryl-esterase (PDB code 1VA4) and Est16 was generated using the Maximum Likehood method (MEGA6.0) with a bootstrap of 1,000 replicates. The estimated value of the shape parameter for the discrete Gamma Distribution is 16.1381. Substitution pattern and rates were estimated under the WAG model (+G) with 5 categories. (B) The amino acid sequence alignment of Est16 with Anti-Kazlauskas lipase (patent number US20050153404) and aryl-esterase (PDB code 1VA4). The residues involved in the substrate-pocket and family classification are shown in bold and the catalytic triad residues are denoted with asterisks (*). The consensus motif of family V is shown.