Fig_4.tif (2.63 MB)
Nitric oxide detection by EPR in P. patens protonema growing in high nitrate.
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posted on 2015-03-05, 02:48 authored by Rigoberto Medina-Andrés, Alejandro Solano-Peralta, Juan Pablo Saucedo-Vázquez, Selene Napsucialy-Mendivil, Jaime Arturo Pimentel-Cabrera, Martha Elena Sosa-Torres, Joseph G. Dubrovsky, Verónica Lira-RuanThe EPR assay was performed as in Fig. 3 using plants that grew for seven days on A) Knop media, B) media added with 8.4 mM Ca(NO3)2, D) Knop media added with 30μM sodium tungstate and E) media added with 8.4 mM Ca(NO3)2 and 30μM sodium tungstate. EPR spectra were recorded at approximately 9.8 GHz (X-band) and 100 kHz modulation (Microwave frequency, 9.777400 GHz for B) and microwave power, 2.0 mW; modulation amplitude: 0.3 mT; T, 297 K). Each graph is a representative spectrum of four independent experiments. C) represents the simulated spectrum of B).
