Fig_3.tif (1.05 MB)
Nitric oxide detection by EPR in P. patens protonema.
figure
posted on 2015-03-05, 02:48 authored by Rigoberto Medina-Andrés, Alejandro Solano-Peralta, Juan Pablo Saucedo-Vázquez, Selene Napsucialy-Mendivil, Jaime Arturo Pimentel-Cabrera, Martha Elena Sosa-Torres, Joseph G. Dubrovsky, Verónica Lira-RuanA) Plants grew for seven days on simple Knop media or C) on medium supplemented with 30 μM sodium tungstate. Homogenized tissues were mixed with (MGD)2Fe(II) and incubated for 1h. EPR spectra were recorded at approximately 9.8 GHz (X-band) and 100 kHz modulation (Microwave frequency, 9.776915 GHz for A and 9.779062 GHz for C; microwave power, 2.0 mW; modulation amplitude: 0.3 mT; T, 297 K). Each graph is a representative spectrum of four independent experiments. B represents the simulated spectrum of A.
