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Identity and purity of mitochondrial fraction.

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posted on 2015-07-06, 06:16 authored by Kai Li, Yong-Chun Cui, Hong Zhang, Xiao-Peng Liu, Dong Zhang, Ai-Li Wu, Jian-Jun Li, Yue Tang

Isolated mitochondria, cytosol were analyzed to check for contamination. (A) Mitochondria were corroborated by transmission electron microscopy. (B) Mitochondrial fractions were labelled with MitoTracker green and analyzed under a fluorescence microscope. (C) Mitochondrial, nuclear, and cell debries were labelled with MitoTracker green and propidium iodide, then analyzed on a flow cytometer. Fluorescence intensity in arbitrary units. (D) Western blot of the same fractions with antibodies for characteristic proteins of mitochondria (VADC1), cytosol (GAPDH) and nuclei (Histone-H3).

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