Effects of Mpe on T-bars and mitochondria in antennal lobes and thoracic ganglia.

Transmission electron microscopy (TEM) images of T-bars and mitochondria inside antennal lobes (ALs) of wild type (WT), untreated PINK1^B9 and in Mpe (0.1%)-treated PINK1^B9 flies. (A): T-bars in a presynaptic bouton of PINK1^B9 ALs (arrowheads). Asterisks indicate mitochondria inside presynaptic boutons and neurites. Inset: high magnification of two T-bar in coronal section. (B): T-bars in presynaptic boutons of ALs of PINK1^B9 Mpe 0.1% (arrowheads). Asterisks indicate mitochondria inside presynaptic boutons and neurites. (C): swelling on the external mitochondrial membrane (at high magnification in the inset) and mitochondrial cristae widely degenerated (asterisks) in ALs of PINK1^B9. (D): Mitochondria of PINK1^B9 Mpe 0.1% (asterisks). (E): Presynaptic T-bar density in ALs of WT, PINK1^B9 and PINK1^B9 0.1% Mpe flies. Values are average + SEM. *indicates p<0.01 at two tailed t-test with respect to PINK1^B9. (F): T-bar density in thoracic ganglia of WT, PINK1^B9 and PINK1^B9 0.1% Mpe flies. Values are average + SEM. *indicates p<0.01 at two tailed t-test with respect to PINK1^B9. (G): Percentages of damaged mitochondria in ALs of WT, PINK1^B9 and PINK1^B9 0.1% Mpe flies. Values are average + SEM. *indicates p<0.01 at two tailed t-test with respect to PINK1^B9. Abbreviations: postsyn: postsynaptic; presyn: presynaptic. Scale bars are 200 µm in A and B and 500 µm in C and D.