Effects of Mpe and L-Dopa on climbing activity.

(A): Climbing activity of adult males wild-type (WT), untreated PINK1^B9, PINK1^B9 treated with Mucuna pruriens extract (Mpe) 0.1% and PINK1^B9 treated with L-Dopa 0.01% (L-Dopa 0.01%). (B): Climbing activity of PINK1^B9 adult males treated with Mpe 1 and 10% as compared with WT and untreated PINK1^B9. (A) and (B): Treatments were administered to flies from their larval stage to the end of their life-cycle (L⁺/A⁺) and their effects were assayed at three different age steps (I: 3–6; II: 10–15; III: 20–25 days) of flies’ life-span. Values are average + SEM. *indicates p<0.05 at two-way ANOVA followed by HSD post-hoc test as compared to WT; **indicates p<0.05 at two-way ANOVA followed by HSD post-hoc test as compared to PINK1^B9; ***indicates p<0.05 at two-way ANOVA followed by HSD post-hoc test as compared to PINK1^B9 Mpe 0.1%. (C) and (D): Percentages of adult males WT, PINK1^B9, Mpe 0.1%, L-Dopa 0.01% (C) and Mpe 1 and 10% (D) that could climb unto, or above, the line drawn at 6 cm from the bottom of the tube within 10 seconds.