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Comparison of the novel rodent hepaciviruses with other Flaviviridae.

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posted on 2013-06-20, 01:58 authored by Jan Felix Drexler, Victor Max Corman, Marcel Alexander Müller, Alexander N. Lukashev, Anatoly Gmyl, Bruno Coutard, Alexander Adam, Daniel Ritz, Lonneke M. Leijten, Debby van Riel, Rene Kallies, Stefan M. Klose, Florian Gloza-Rausch, Tabea Binger, Augustina Annan, Yaw Adu-Sarkodie, Samuel Oppong, Mathieu Bourgarel, Daniel Rupp, Bernd Hoffmann, Mathias Schlegel, Beate M. Kümmerer, Detlev H. Krüger, Jonas Schmidt-Chanasit, Alvaro Aguilar Setién, Veronika M. Cottontail, Thiravat Hemachudha, Supaporn Wacharapluesadee, Klaus Osterrieder, Ralf Bartenschlager, Sonja Matthee, Martin Beer, Thijs Kuiken, Chantal Reusken, Eric M. Leroy, Rainer G. Ulrich, Christian Drosten

A. Bayesian phylogeny of the Flaviviridae NS5B gene. The analysis was done in MrBayes and included representatives of all Flaviviridae genera and those five novel rodent viruses whose full polyprotein could be determined. The WAG amino acid substitution model was used. Statistical support of grouping from Bayesian posterior probabilities and 1,000 parallel Maximum Likelihood bootstrap replicates is indicated at deep node points. Scale bar corresponds to genetic distance. A tombusvirus (Lisianthus necrosis virus, GenBank accession number NC_007983) was chosen as an outgroup. B. Amino acid distance of the complete NS5B gene within Flaviviridae clades. Maximum amino acid sequence distance was calculated with MEGA5 using the pairwise deletion option and all Flaviviridae members contained in panel A. The dotted line indicates 70% distance for clarity of graphical presentation only.