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Cell cycle analysis of SCLC-24H cells.

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posted on 2014-02-28, 03:00 authored by Tamara Murmann, Carmen Carrillo-García, Nadine Veit, Cornelius Courts, Alexander Glassmann, Viktor Janzen, Burkhard Madea, Markus Reinartz, Anne Harzen, Michael Nowak, Sven Perner, Jochen Winter, Rainer Probstmeier

Cells were either cultured in the absence or presence of serum (Serum); treated for 24 h without (−) or with different SSP concentrations (20, 50 nM) (SSP+); or treated without SSP for 48 h (−) or with 50 nM SSP for 24 h (SSP +/−) and then analyzed immediately (+) or cultured for further 24 h in the absence of the drug (+/−). Representative FACS plots of cell viability (Annexin V; left panels) and cell cycle progression (Ki67; right panels) under different treatment conditions are shown.

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