S1_Fig.tif (803.95 kB)
Effect of high nitrate on P. patens protonema growth and NR activity.
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posted on 2015-03-05, 02:48 authored by Rigoberto Medina-Andrés, Alejandro Solano-Peralta, Juan Pablo Saucedo-Vázquez, Selene Napsucialy-Mendivil, Jaime Arturo Pimentel-Cabrera, Martha Elena Sosa-Torres, Joseph G. Dubrovsky, Verónica Lira-RuanThe effect of high nitrate in growth A) and NR activity B) was analyzed in plants that grew for seven days in Knop media alone or with 8.4 mM Ca(NO3)2, supplemented or not with 30μM sodium tungstate. Relative growth rate was measured according the formula: (ln af—ln a0)t-1 where af and a0 are the plant area at day 7 and at day 0, respectively. t is the time growth in days = 7. Data are mean of three independent experiments. Data were analyzed by one-way ANOVA and Tukey’s multiple comparison test (n = 56 in panel A and n = 7–9 in panel B). Different letters indicate a statistically significant difference (P < 0.05). Error bars denote SE.
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