PCR Sprint Programmed T vs Recorded T

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Salvagno, Anthony (2012): PCR Sprint Programmed T vs Recorded T. figshare.

Retrieved 10:09, Nov 25, 2015 (GMT)


I put the machine in manual mode so I can change the T when I needed to and I would take data points at 30 seconds for melting and annealing temperatures and at 30s and 5 minutes for extending T. This is how long I would be doing that step during the PCR reaction so it seemed to make sense. Attached is a chart of the Set T and the recorded T at each interval, the raw data of recorded T, a converted recorded T excel file, and the plot of that data.


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