E. Coli Growth over 4 hours

None
None
None
None
None
Share this:
Embed*
Cite this:

Salvagno, Anthony; haddad, alexandria (2012): E. Coli Growth over 4 hours. figshare.

http://dx.doi.org/10.6084/m9.figshare.91671
Retrieved 01:24, Oct 25, 2014 (GMT)

Description

In preparation for some deuterium effects on E. coli and S. cerevisiae, I grew a starter culture and diluted it in 3 different concentrations. 1:10, 1:5, and 1:2. These dilutions were then grown at 37C for 4 hours and an absorption measurement was taken every hour. This fileset contains the raw data and some played with data, along with some figures made in Excel from the data.

The file labeled "arb-ecoli-growth.png" is a figure made from manipulated data. I tried to combine the three data sets into one graph to see if I could extract some sort of growth information. I'm pretty sure I didn't do it right, but I included the image here nonetheless.

In the 1:10 dilution sample, the cells would double in slightly less than one hour, every hour. In the 1:2 dilution, the growth rate was much slower, and the growth rate seemed to peak rather early in the trial. The 1:5 dilution is an overlap of growths between both the 1:10 and 1:2 dilutions. I don't know what to make of that.

Also included in the fileset is an image of the absorbance spectrum from the nanodrop for every sample (including blanks taken every hour).

Links

Comments (0)

You must be logged in to post comments.

Last saved: 2012-04-20

Cite "Filename"

Place your mouse over the citation text to select it

Embed "E. Coli Growth over 4 hours"

Place your mouse over the embed code to select and copy it

Claim article

You claim request was sent. I will be handled in the next 24 hours.

Close window

Feedback

We appreciate all your comments, questions, suggestions or gratitude.

Login

The username or password entered are wrong.

Reset password

Your password will be sent to your registered e-mail address.

Create account

I agree to the Terms & Conditions *