gnpl_a_895730_sm6000.docx (2.89 MB)
A new cytotoxic steroid from co-fermentation of two marine alga-derived micro-organisms
Version 3 2014-08-18, 13:17
Version 2 2014-08-18, 13:17
Version 1 2014-08-18, 00:00
journal contribution
posted on 2014-08-18, 13:17 authored by Sherif S. Ebada, Thomas Fischer, Sarah Klaßen, Alexandra Hamacher, Yoen Ok Roth, Matthias U. Kassack, Eckhard H. RothBioactivity-guided chemical investigation of a co-culture of marine-derived micro-organisms has yielded one new steroid, 7β-hydroxycholesterol-1β-carboxylic acid (1) with an unprecedented carboxylic acid group at C-1, together with three known steroidal metabolites (2–4). The chemical structures and stereochemistry of the isolated compounds were unambiguously determined based on extensive 1D, 2D NMR and HR-ESI-MS measurements. The isolated compounds were assessed for their cytotoxic activity against four different human tumour cell lines K562 (leukaemia), HCT116 (colon), A2780 (ovary) and its cisplatin-resistant mutant (A2780 CisR), and they revealed moderate activities with IC50 values ranging from 10.0 to 100.0 μM.