PCR Sprint Programmed T vs Recorded T Anthony Salvagno 10.6084/m9.figshare.94414.v1 https://figshare.com/articles/dataset/PCR_Sprint_Programmed_T_vs_Recorded_T/94414 <p>I put the machine in manual mode so I can change the T when I needed to and I would take data points at 30 seconds for melting and annealing temperatures and at 30s and 5 minutes for extending T. This is how long I would be doing that step during the PCR reaction so it seemed to make sense. Attached is a chart of the Set T and the recorded T at each interval, the raw data of recorded T, a converted recorded T excel file, and the plot of that data.</p> 2012-08-23 18:51:49 equipment pcr thermal cycler Molecular Biology