Doubling times of HDPSCs in basal or osteogenic media HahnelMark 2011 <p>HDPSC populations were cultured in DMEM.10% (Basal) or osteogenic media. It can be seen that doubling time changes as the duration of culture increases. From the graph, it is possible to establish that the most efficient dt, and therefore the optimum expansion rate, occurs between days 6 and 9 of culture. This is true for both types of media.</p>